商品描述:
商品屬性
商品描述:
商品屬性
己糖激酶
中文名稱: 己糖激酶
其他中文名稱: 己糖磷酸激酶
英文名稱: HK
其他英文名稱: Hexokinase from Saccharomyces cerevisiae;ATP:D-Hexose-6-phosphotransferase;Hexokinase from yeast
產(chǎn)品規(guī)格: BR,~100u/mg
己糖激酶包裝: 500U/1KU/2.5KU/10KU
CAS號(hào):9001-51-8
級別:BR
分子量:~ 54 kDa (monomer);~110 kDa (dimer)
活力:~ 100 units/mg protein
活力定義:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH:7.5~9.0
己糖激酶性狀:白色粉末。釀酒酵母提取。催化劑(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;抑制劑(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途:生化研究。為催化己糖6位上由ATP使之產(chǎn)生磷酸化反應(yīng)的酶。對α-D葡萄糖的親和性很高(Km=1×10-5M),對其他的己糖也可進(jìn)行磷酸化。此反應(yīng)的平衡向右偏移?!鱃°’=4.0千卡。該酶可被Mg2 、 K 所活化,但可被HS試劑阻抑。
保存:-20℃